universal ivf medium  (ORIGIO Inc)

Journal: F&S Reports

Article Title: Assisted sperm fusion insemination improves fertilization rates and increases usable embryos for transfer: a clinical sibling-oocyte study

doi: 10.1016/j.xfre.2024.11.006

Figure Lengend Snippet: Assisted sperm fusion insemination procedure (representative images). ( A, B ) Inserting the injection pipette into the perivitelline space by penetrating the zona pellucida. ( C ) Pressing the head of the motile sperm onto the oocyte membrane for 10 seconds. ( D ) Adhesion of the sperm head to the oocyte membrane while the tail is moving. The bottom right corner of Figure D is an enlarged image of sperm adhesion. Green arrows: motile sperm collected from the zona pellucida.

Article Snippet: The retrieved oocytes, including MII, immature, and degenerated oocytes, were incubated in 1.0 mL of the insemination medium (Universal IVF Medium; CooperSurgical, Trumbull, CT) that had been equilibrated in a 2-well dish (2-well well dish for in vitro fertilization; Nakamedical, Tokyo, Japan) for 2–3 hours at 37 °C in 6% CO 2 and 5% O 2 .

Techniques: Injection, Transferring, Membrane

Journal: F&S Reports

Article Title: Assisted sperm fusion insemination improves fertilization rates and increases usable embryos for transfer: a clinical sibling-oocyte study

doi: 10.1016/j.xfre.2024.11.006

Figure Lengend Snippet: Comparison of morphokinetic parameters between assisted sperm fusion insemination and conventional intracytoplasmic sperm injection.

Article Snippet: The retrieved oocytes, including MII, immature, and degenerated oocytes, were incubated in 1.0 mL of the insemination medium (Universal IVF Medium; CooperSurgical, Trumbull, CT) that had been equilibrated in a 2-well dish (2-well well dish for in vitro fertilization; Nakamedical, Tokyo, Japan) for 2–3 hours at 37 °C in 6% CO 2 and 5% O 2 .

Techniques: Comparison, Injection